宏基因组测序技术在未知病原体感染以及危重症感染诊断中的临床应用价值日益凸显。由于宏基因组测序庞大的数据量以及临床诊疗的复杂性,使得宏基因组测序在实际应用中存在着数据分析困难、不易解读的难题。因此,在临床实践的过程中,把握生物信息学分析的关键点、建立标准化的生物信息学分析流程至关重要,这是宏基因组测序由实验室走向临床转化的重要步骤。 目前,宏基因组测序的生物信息学分析已经有了长足的进步,但随着临床对生物信息学分析规范化、标准化的高要求以及计算机技术的发展,宏基因组测序的生物信息学分析也面临着新的挑战。本文主要从质量控制、致病菌的判定以及可视化等方面进行阐述。
一、mNGS生物信息学分析概况 二、mNGS生物信息学分析的临床应用关键点
三、mNGS生物信息学分析在临床应用中的展望
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